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Samples were resuspended in Tris-EDTA buffer containing 1 mg ml−1 boiled bovine pancreatic RNAse A, incubated for 1 h at 40°C, and finally mixed with DNA sample buffer.
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Bovine bones were boiled for 12 h to remove the attached flesh and fat.
Controls comprising apolipoprotein A1, bovine serum albumin, trypsin inhibitor, and boiled apoE had no effect, demonstrating specificity of the apoE effect.
Frozen samples were sonicated in boiling 1% SDS containing 50 mM NaF and boiled for an additional 5 min. Protein concentrations were determined by the BCA protein assay (Pierce) using bovine serum albumin standards.
Boiled eggs.
Best boiled.
He boiled.
Boiled Weeds?
Boiled Ham.
Boiled food.
Membranes were boiled in 50 mL phosphate buffered saline by microwaving for 25 s, followed by a 4 min wait, then again for 15 s and blocked with 5% bovine serum albumin (Sigma-Aldrich) for 1 h at room temperature.
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