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Add stock and boil until reduced by half then add cream and boil for further five minutes.
Mix thoroughly; leave it to boil for further 5 minutes.
Boil the stock, put in the pods, and boil for a further 5 minutes.
Bound proteins were eluted from the beads with 1XSDS sample buffer for 10 min at RT and the supernatant was transferred to a new centrifuge tube to boil at 95 °C for further 2 min before SDS-PAGE analysis.
Cells were separated from the medium by centrifugation through dinonyl phthalate and were then immediately dissolved in SDS and β-mercaptoethanol with protease and phosphatase inhibitors, frozen within 10 seconds and thawed in boiling water for further analysis [ 43].
All herbs were soaked in water in a ratio of 1/3 (g/ml) for 30 min and subsequently boiled for a further 30 min. After saving the supernatant containing constituent extracted from the herbs, the same volume of water was added to the dregs and boiled for a further 30 min. The two extracts were pooled together and concentrated by means of heating evaporation.
The samples were then boiled for a further 5 min, cooled to room temperature and sonicated for 30 seconds to fragment DNA contained in the cell lysate.
The solution was further boiled for 15 min and cooled.
The mixture was further boiled for about 60 min and the color of the solution turned from colorless to yellow within 10 min.
The extract was further boiled for 10 min and sieved and filtered twice by using filter paper No. 42 (Whatman, USA).
The mixed solution was kept boiling for a further 8 min. Finally, the green-gray solution was obtained which was stable for several days or weeks.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com