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Boil for an additional 15 minutes.
Continue to boil for an additional 5 minutes.
Add in the sugars, spices, and butter and boil for an additional minute, stirring constantly.
Boil them for 30 seconds to a minute on one side and then flip them over and boil for an additional 30 seconds to one minute on the other side.
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The solution was kept boiling for an additional 15 min. Then the solution was cooled to room temperature and filtered, the AuNPs were obtained and stored at 4°C for further use.
The remaining ethoxymethylenemalononitrile was added, at such a rate to maintain gentle boiling of the solution, after all the ethoxymethylenemalononitrile had been added, the solution was gently boiled for an additional 30 min and finally was set aside overnight in the refrigerator.
Frozen samples were sonicated in boiling 1% SDS containing 50 mM NaF and boiled for an additional 5 min. Protein concentrations were determined by the BCA protein assay (Pierce) using bovine serum albumin standards.
Cell lysates were boiled for an additional 20 min and stored at −20°C.
The sample was boiled for an additional 10 min and then centrifuged at 15,000× g for 15 min at room temperature.
The reaction mixture was maintained at the boiling temperature for an additional 3.5 min followed by cooling to room temperature.
Tubes were immersed in a boiling water bath for 5 min, sonicated for 5 min in a Branson 1210 bath sonicator (Branson Ultrasonic Corporation, Danbury, CT), incubated at room temperature for 45 min, and returned to boiling water for an additional 5 min to fully solubilize the tissue sample.
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CEO of Professional Science Editing for Scientists @ prosciediting.com