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The ability to attenuate accelerations through the upper body was quantified using the attenuation coefficient.
Completely balanced retention of Ir activity retained in the body was quantified by gamma spectroscopy in a 1-L well-type scintillation detector (Eurisys/ Canberra, Rüsselsheim, Germany) corrected for background radiation and detector geometry and sensitivity.
The mean GFP intensity throughout the body was quantified from captured images of multiple flies using Image J software, and this analysis confirmed the up-regulation of both reporters during aging, in both males and females.
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In addition, lesional metabolically active tumor volume (MAV) and total MAV (defined as the sum of lesional MAV among all lesions in the body) were quantified and used to calculate lesional metabolic volumetric product (MVP) (defined as the product of lesional MAV and lesional SUV) and total MVP (defined as the sum of lesional MVP among all lesions in the body).
The number of cells bearing neurites longer than 1.5 cell bodies was quantified relative to the total number of neurons counted in at least 10 random fields of three different wells in each experiment.
The number of RGC cell bodies was quantified in areas of 62 500 μm at 1 mm and 1.5 mm from the optic disk.
Briefly, NPCs were stained with 1 μg/ml Hoechst 33342 (Sigma) and the fraction of cells exhibiting an apoptotic nuclear morphology characterized by chromatin condensation and/or apoptotic bodies was quantified.
Basal and isoproterenol-stimulated cyclic AMP concentrations in iris ciliary bodies were quantified by radioimmunoassay techniques in the presence and absence of ICI and spiradoline.
Asbestos bodies are quantified by light microscopy whereas concentrations of coated and uncoated asbestos fibers are determined by means of scanning electron microscopy, with the results compared to a well-defined control population.
PK-resistant aggregates in neuronal cell bodies were quantified by counting of individual aggregates.
Only close appositions to Cx45-eGFP cell bodies were quantified since there is only sparse dendritic staining for eGFP.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com