Totally, 35,191 ESTs from ten kinds of materials (i.e., embryo, brain, compound eye, epidermis, fat body, middle silk gland, posterior silk gland, midgut, ovary and testis from larvae) were blasted against our assembled contigs with blastn, and 18,978 (53.9%) of ESTs showed ≥ 90% identity with more than 50% matched length of the corresponding ESTs.
We have sequenced miRNAs in the whole body and silk gland of the silkworm.
Additionally, we examined the developmental patterns of miRNA expression during metamorphosis of the body wall, silk glands, midgut and fat body.
Periods of rapid morphological change were associated with alterations in miRNA expression patterns in the body wall, silk glands, midgut and fat body during metamorphosis.
The two BmTOR genes have similar expression patterns and tissue distribution in fat body, midgut, silk glands, and other organs, levels being highest during molting and pupation.
In this study, we established the distribution of silkworm miRNAs in fifth-instar day 3 larvae and their spatiotemporal expression patterns in the body wall, silk glands, midgut and fat body during metamorphosis.
To determine the extent of tissue-specific changes during specific developmental events, we assessed changes in miRNA expression in four individual tissues and organs (body wall, silk glands, midgut and fat body) from fifth-instar day 7 larvae to pupae.
And it even showed up as an all-in-one body sock over silk taffeta shorts, but only Lady Gaga would take that particular look out for a spin.
B.mori: Bombyx mori; miRNA: microRNA; 3'UTRs: 3'untregionsed regions; HD: head; BW: body wall; SG: silk gland; ASG: anterior silk gland; PSG: posterior silk gland; MG: midgut; FB: fat body; OV: ovary; TE: testis; HC:hemocyte; MT: malpighian tubule; GN: gonad; f: female; m: male; SAM: Significance Analysis of Microarrays.
