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Drug administration at time points after 4 hpf had no effect on the A-P body axis, consistent with an early role for FGF-MAPK in establishing body length.
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This keeps the information about the tangent plane and the optical axis consistent with WCS.
This axis (D) is connected to the body axis (B) with a universal (or Cardan) joint to allow angulations of axis (D) relative to the body axis (B).
In zebrafish embryos, we observed pericardial edema and dorsal curvature of the body axis associated with BaP.
Dots mark 3 positions along the body axis, with head direction indicated by the larger circles.
We rotated the new coordinate system around the z-axis until the x-axis was parallel to this body axis, with the ThC joints of the front legs having a larger x-value than the rear legs.
For freehand preparation the specimens were halved along the body axis with a razorblade.
Regardless of the speed, stance traces run parallel to the body axis with the forelegs positioned more medially (Fig. 4a).
The latter vary along the longitudinal body axis with respect to the number of axons they comprise.
Authors' response: We inserted the following into the text: "...a recent study showed the Wnt expression pattern in the demosponge Amphimedon queenslandica to be consistent with a role in patterning the primary body axis during development[ 57]." This reviewer provided no comments for publication.
Firstly, components of the Wnt pathway have been found in two species of demosponge [ 9, 56] and, a recent study showed the Wnt expression pattern in the demosponge Amphimedon queenslandica to be consistent with a role in patterning the primary body axis during development[ 57].
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