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In prosperous countries, large numbers of people – whether they wanted to lose weight or not – became understandably scared of the modern food supply and what it was doing to our bodies: type 2 diabetes, obesity and cardiovascular disease, not to mention a host of other complaints that are influenced by diet, ranging from Alzheimer's to gout.
To determine the localization of V-ATPase subunits a1 and B1/2, equal amounts of proteins isolated from lung plasma membranes, lamellar bodies, type II cells and lung homogenates were immunoblotted exactly as described above.
Immunolocalisation studies of E. tenella-infected chicken intestine using this antibody revealed reactivity against wall-forming bodies type I of macrogametes, with GAM56 antibody used as a counterstain for wall-forming bodies type II (WFBII).
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