Randomly picked blunt end-cloned small insert pUC19 vector-based plasmids (average ∼3 kb insert size) were sequenced at both ends using universal forward and reverse sequencing primers, according to standard protocols established at the University of Washington Genome Center.
"Who Told You" picked up the blunt beat and plinking synthesizer of Detroit ghetto-tech, with MC Dynamite rapping in sync with the bass drum; "Lucky Pressure" had Onallee singing rhythm-and-blues verses and refrains over a backdrop of melancholy string-orchestra chords and the fast beat.
I haven't picked up a blunt or listened to Phish since.
For matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF), three plugs of the band of interest from a 1-D gel were picked with a blunt-cut 20 gauge needle and deposited in a 96-well tray of an automated digester according to the procedures recommended by ProGest (Genomic Solutions).
I slid a chain-mail glove over my left hand, picked up a short, strong, blunt oyster-shucking knife with my right, and went to work.
These rebellious, antifashion messages, blunted over decades of exposure, have been picked up, inevitably, by the world of high style.
And after passing one blunt, dirty needle around 30 men, some have picked up new diseases.
The resulting PCR products were subcloned into the holding vector pSC-B (StrataClone Blunt PCR Cloning Kit, Agilent Technologies) and twelve colonies (white) picked for each clonal line.
The headlines came blunt and quick on the news channels, moments after Bresnan picked up Sreesanth and put an end to India's agony.
"Let's be blunt: The drama-club kids and the artsy, creative kids often get picked on just as badly as the gay kids".
Then she picked up a magazine and glanced through it, cutting the pages with the blunt edge of her knife.
