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Moreover, the blue substrate glaze was revealed to be an alkali glaze coloured by cobalt.
Furthermore, the gilding decorations were suggested to be achieved by firing gold powder on the pre-fired blue substrate glaze.
The developing reagents were DAB ZLI-90188, ZSGB-BIO) and VECTOR BLUE SUBSTRATE KIT SK-53000, Vector Laboratories , Inc.
AFM, SEM and optical microscopy proved that the gold flakes partially penetrated the pre-fired blue substrate glaze.
Moreover, AFM observations proved that the gold flakes were immersed in the blue substrate glaze (Figure 4).
Potassium, calcium, titanium, manganese, lead, cobalt and arsenic appeared in the μ-XRF analysis of the golden layer are most certainly derived from the blue substrate glaze.
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After incubation for 1 hour at RT, the plates were washed and 100 µL of Enhanced K-Blue substrate (Neogen Corp., Lexington, KY) was added to detect bound antibodies.
K-Blue substrate was from Neogen (KY, USA).
Culture supeRNAtants were harvested 24 h post transfection and assayed for alkaline phosphatase activity by incubating with the QUANTI-BLUE substrate (Invivogen) and absorbance was measured at 625 nm using a spectrophotometer.
Following this, the cells were washed three times in TBS and net changes in HA-tagged receptor cell surface expression were determined colorimetrically at 650 nm using the K-Blue substrate (Neogen Corp) as previously described [16].
Thp1-Blue cells (InvivoGen) expressing TLR1/2, TLR2, TLR2/6, TLR4, TLR5, TLR8, NOD1, and NOD2 were stimulated with Adjuplex or PRR ligands (InvivoGen) for 24 h, and the supernatants were tested for secreted embryonic alkaline phosphatase (SEAP) expressed following NF-κB or AP1 activation, using QUANTI-Blue substrate (InvivoGen).
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