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The resulting blue solution was mixed and shaken with equal volume of diethyl ether for 2 min.
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Equal volumes of cell suspension and 0.4% (w/v) trypan blue solution were mixed and incubated at room temperature for 5 min. Total cell and dead cell, trypan blue positive, counts were performed with a hemocytometer using a light microscope.
(B ) As described in (A ), but 1 part of Marina-Blue-PE (0.2 mol%) mixed micellar solution was mixed with 0 5 parts of NBD-PE (3 mol%) mixed micellar solution prior to liposome formation.
A total of 10 μL of this solution was mixed with 190 μL of Trypan blue (Sigma-Aldrich, St . Louis MO) to distinguish live cells from dead cells and to facilitate cell counting.
Then solution was mixed.
The solution was mixed overnight at 25 °C.
Then this solution was mixed with above solution.
The solution was mixed by pipetting.
The solution was mixed thoroughly at 0 °C.
The BSA or TG solution was mixed with an equal volume of sample buffer (125 mM Tris HCl, pH 6.8; 4 % SDS; 20 % glycerol; 0.2 M DTT; and 0.0125 % bromophenol blue).
were added, and this solution was mixed again.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com