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Lastly, Loffler's alkaline methylene blue solution was made by dissolving 2 g of methylene blue (Ziyi Reagent Company, Shanghai, China) into 100 mL of 95% ethanol (Ziyi Reagent Company, Shanghai, China) to make 100 mL of saturated solution.
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This contrast solution was made by adding 0.4 ml of gadodiamide (Omniscan®, GE Healthcare Inc., Glattbrugg, Switzerland) and 0.05 g of toluidine blue to 100 ml of 0.9% NaCl.
The denaturing solution was made in a ca. 25-mL total volume containing 23.75 mL 99% formamide, 1.25 mL 1% (w v−1) xylene cyanol, and 10 mg bromophenol blue.
Stock solutions were made in dimethyl sulfoxide.
Standard solutions were made using 30%H2O22.
Two sets of stock solutions were made.
It contained protein since the color of Coomassie Blue solution was turned into blue.
Methylene blue solution was used to aid visualization by blue color.
Trypan blue solution was from GE Healthcare Life Sciences (Little Chalfont, UK).
Methylene blue solution was obtained from Sigma-Aldrich.
Bromophenol blue solution was prepared directly before use.
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