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(B) HAI-178-FMNPs bound to the membrane of MGC803 cells, blue nucleus (DAPI staining) (×10).
(A-C) CLR- (red) and (D-F) RAMP1-immunostaining (green) combined with DAPI (blue) nucleus staining.
In contrast, no green dots of Fe3O4@SiO2-Amino NPs were detected in the blue nucleus district.
e Formation of clamp connection, through which a blue nucleus migrates back to the penultimate cell, while two new septa are formed in the clamp cell and hyphal tip.
IM in yellow and the FC on the left, loses the body to leave only the blue nucleus.
Overall endopolyploidy level, which is determined by the average number of DAPI stained blue nucleus per cells was found to be significantly reduced (28%) in AGO2 knocked down cells.
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Live cells with uniformly light blue nuclei were treated with Hoechst 33258 [14–17] and observed under a fluorescence microscope.
Thresholding of the blue (nuclei stained with DAPI) and red fluorescent area (nuclei stained with PARP1) was performed using MetaMorph® Software (Molecular Devices, Sunnyvale, CA).
Hoechst 33258 is a hydrophilic and fluorescent probe only when bounded the DNA of cells, and live cells with uniformly light blue nuclei were treated with Hoechst 33258 [33258
The image shows that the coumarin-6-loaded PEG-PLGA nanoparticles (green) have entered into cytoplasm, and the blue nuclei stained by DAPI indicates that the coumarin-6-loaded PEG-PLGA nanoparticles are situated around the nuclei (blue, DAPI) and normal nuclei.
The software identified positive cells by combining two masks where areas of a nucleus surrounded by a red-brown staining are identified as positive cells, and isolated blue (nuclei without staining) or red are ignored.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com