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A sample of 100 μL of the supernatant from each specimen was analyzed in a UV-Visible spectrophotometer to determine the amount of methylene blue leakage.
TBTC exposure in-vivo resulted in increased tin content in the testis with enhanced Evans blue leakage into the testicular tissue indicating blood testis barrier disruption.
At the lower dose that is currently in clinical use (4 mg/kg beginning at 3 h), hemin also improved barrier function in both models, as assessed by both Evans blue and FITC-dextran leakage; however, it was somewhat less potent, reducing Evans blue leakage by about half.
Quantitative analysis showed that, in comparison with wild type controls, the area with Evans blue leakage in brain parenchyma was dramatically increased (P ≤ 0.001 vs. WT).
Fig. 3 Absolute values of the full-loop (lowermost, blue), leakage (middle, red) and total (uppermost, black) magnetic fields at a point of observation located at 5 km east of a south-north railway as functions of time produced by a train travelling from south to north.
Representative mouse skin patches showing Evan's blue leakage in MCP-1 injection spots are presented in Figure S3.
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Red: Block diagonalization; blue: leakage-bounded.
Cerebrovascular permeability was detected by Evans blue dye leakage in MCAO rats.
As shown in Figure 2(a), supplementation of α-MG increased OBF and decreased Evan blue dyes leakage.
As shown in Figure 2A-D, Evan's blue dye leakage was observed in both SRC-3-/ and wild-type mice.
This impaired continuity was also confirmed by observing trypan blue dye leakage in Apc CKO lungs after its intracardiac injection (data not shown).
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