Single anthers were mounted in staining buffer (1xPBS, 5% DMSO, and 0.01% Tween 20) containing combinations of 0.1 mg/mL propidium iodide, 2 mg/mL DAPI, or 0.01% (w/v) aniline blue and inspected in a Zeiss Axioplan2 imaging microscope equipped with epifluorescence.
Semithin sections were stained with toluidine blue and inspected in Eclipse E400 (Nikon) light microscope.
The trained operator should also be able to detect the slight blue colored ovaries from females injected with 0.4% Trypan blue, which was inspected under an optical dissecting microscope.
They are clad in blue jeans and inspected like cattle by men with pistols and crew cuts and gray uniforms.
When he pried the lids off the yellow, pink and blue boxes and inspected the frames, Mr. Harvey found very little activity inside.
The older girls took charge of her and inspected the blue rabbit while Rose made another call on her car.
Mr. Lazio, on the second day of a bus tour across the state, watched machinery spool cable wire at a plant in Cortland and then donned a white protective coat and blue ducks as he inspected components for motion sensors at a company in Ithaca.
Methyl viologen was added to all preparations to a final concentration of 150 mg/L, incubated anaerobically at 55 °C for five days and visually inspected for blue coloration as an indication of reduced methyl viologen.
At a table on the side of the ticket counter, federal agents searching a blue duffle bag not only inspected all the shoes and clothing, but also swiped deodorant sticks, shampoo bottles and the cap and container of an Extra Strength Tylenol bottle, checking each for explosives.
The collected organisms were inspected with a blue flashlight (BlueStar, NightSea; Andover, MA).
