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The colors refer to the general section of the algorithm where the step is performed: database interpretation (red), mapping (blue), extraction (green) and formalizing the template (yellow).
Each color groups blocks belonging to one sub-task: database interpretation (red), mapping (blue), extraction (green) and post-processing (yellow) Fig. 6 Illustration of the identification of a reactive center for the Diels Alder reaction.
Thereafter mice were sacrificed and tumors were excised, dried (60°C, 16 hr), and weighed before Evans blue extraction in 1 ml of formamide at 55°C for 16 hr.
These results can explain the significant reduction that was found in retinal edema following angiography or Evan's blue extraction, and also the diminished tissue edema and spongiosis found in the DTH reaction.
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Moreover, the present study demonstrated that all bacterial leakproof specimens leaked when submitted to the blue methylene extraction model.
We determined that we were unrelated: he was of blue-blooded Austrian extraction; I derived from immigrant Romanians.
For real-time PCR analysis, total RNA (1.25 μg) extracted from cells with the use of the easy-BLUE RNA extraction kit (Intron) was subjected to RT with Moloney murine leukaemia virus reverse transcriptase (Invitrogen, Carlsbad, CA, USA).
Total RNA was extracted from cells with the use of the easy-BLUE RNA extraction kit (Intron, Sungnam, Korea) and then was dissolved in diethylpyrocarbonate-treated water and quantified by measuring the absorbance at 260 nm.
Total RNAs were isolated from keratinocytes using Easy-blue RNA extraction kit (Intron, Daejeon, Korea).
Total RNA was isolated using an easy-BLUE RNA Extraction Kit (iNtRON, Sungnam, Korea), and cDNA was prepared using Reverse Transcription Master Premix (ELPisbio, Taejeon, Korea), according to the manufacturers' instructions.
Total RNA was isolated from HaCaT cells according to the manufacturer's specifications using an easy-BLUE RNA extraction kit (INtRON Biotech., Seoul, Republic of Korea).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com