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Media supplemented with AFA, Blue extract or Pure-PC, stimulated the growth of L. acidophilus as compared to the standard MRS broth and enhanced the CFCSs antimicrobial effects.
The aim of this research was to investigate the effect of Aphanizomenon flos-aquae (AFA) and two of its extracts, Blue extract and Pure-PC on Lactobacillus acidophilus DDS-1 growth ability and antimicrobial activity.
The antimicrobial activity of cell-free culture supernatants (CFCSs) of L. acidophilus grown with or without AFA, Blue extract or Pure-PC, was determined against Escherichia coli O157 H7 ATCC 35150 and Candida albicans ATCC 14053 by agar well diffusion and time kill-studies.
The blue extract (2 ml) was vigorously shaken with an equal volume of diethyl ether.
Then, the blue extract was mixed with diethyl ether in a ratio of 1 1.
Four mL of the blue extract was then shaken vigorously with an equal volume of ether.
Similar(51)
The quantity of Alcian blue extracted in μg/grams of the wet colon was then calculated.
The amount of alcian blue extracted per gram of wet glandular tissue was then calculated.
The quantity of mucin was expressed as μg of Alcian blue extracted per weight (g) of wet stomach glandular tissue.
PCR products from nematode homogenates were cloned into pGEM-T Easy (Promega), transformed into E. coli XL1-Blue, extracted, amplified and purified according to standard procedures, and sequenced as described below.
Turquoise Blue extracts generated a number of signals greater than Remazol Black and Novacron Red extracts.
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