The dyes used in this study included amido black, aniline blue, brilliant green, bromocresol purple, bromothymol blue, congo red, crystal violet, erioglaucine, fast green, fuchsin, malachite green, metanil yellow, methylene green, methyl green, methyl orange, methyl red, methyl violet, resazurin, safranin T, toluidine blue O, trypan blue and water blue.
The gel loading mixture was prepared by adding 5% Coomassie Blue Brilliant G-250 dissolved in 1 M aminocaproic acid to the sample at a ratio of 1∶6 (dye:sample volume).
As a loading control, we dyed the gel for 15 min with fixing solution (30% methanol, 10% acetic acid) plus 0.025% Coomassie Blue (Brilliant Blue R, Sigma).
After the reaction was complete, each mixture was separated by 10%% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and stained with 0.1 % Coomassie Blue Brilliant solution.
Bromophenol blue, bromothymol blue, brilliant cresyl blue, thymol blue, phenol red, bromocresol green, and helasol green were applied as 50 mL/100 mL solutions in 2% aqueous sodium hydroxide solution.
New visualizing reagents (aniline blue, alkaline blue, neutral red, brilliant green, bromothymol blue, brilliant cresyl blue, thymol blue, bromophenol blue, phenol red, bromocresol green, and helasol green) were used for the detection of the free fatty acids and their ammonium salts.
That film, which tells the story of a virtuoso jazz drummer and his sadistic orchestra leader, was so out-of-the-blue brilliant that, after seeing it, it was hard not to become its personal publicist, imploring friends, even strangers to see it.
Preparative gels were stained with Roti®-Blue, a colloidal coomassie brilliant blue G250 stain.
The Kings and the Blues looked brilliant in winning their first-round series in five games.
The calibration of the models to the Brilliant Blue observations shows that Brilliant Blue has a solid biomass dependent sorption that is not compliant with the assumed linear constant Kd behaviour.
Gelatinase activity was visualized by staining gels with Coomassie Brilliant Blue G250 (0.25% Coomassie Brilliant Blue G250, methanol 30%, acetic acid 10%) and destained with acetic acid/methanol/dH2O (1:3:6).
