Triple staining methylene blue-azur A-safranin O (MAS), visualising proteins, lipids and starch, was performed to obtain an overview of the embedded tap-root tissue [ 41, 42].
Alternatively, thin sections of 1 µm were stained by a mixture of toluidine blue and azur II and observed under a Leica DME photonic microscope.
The semithin sections were stained with a mixture of 1 : 1 toluidine blue and azur 2. Images were acquired with a light microscope (Olympus BX 51) connected to a digital camera (Olympus Q color 5).
Fourty μm frozen sections were cut and further dissected by JMV, our pathologist, after quick staining with RNAse-free azur blue solution.
(F) Low-power micrograph of a trigeminal ganglion section, stained with azur methylene blue, with the ophthalmic (V1), maxillary (V2) and mandibular division (V3), in which separate cell counts were made.
Asmar, an impetuous, dark-featured youth, sits with his best friend, Azur, a blue-eyed prince, as they marvel at songs of the Djinn Fairy, a legendary creature locked away in sadness beyond enchanted doors and guardian monsters.
Semi-thin sections (1 μm) were stained with azur-methylene blue according to standard methods.
Semithin cross sections of cardiac muscle from control (A) and ATGL (B) animals stained with azur-methylene blue.
Hyphae and conidia were also subjected to HCl-Giemsa stains (Giemsa's azur eosin methylene blue solution, Merck KGaA, Darmstadt, Germany) in accordance with published protocols (Knox-Davies and Dickson 1960; Ward and Ciurysek 1962) with slight modification and is summarized as follows.
Next, cells were rinsed in PBS, fixed with ethanol (100%) and stained overnight with Giemsa's azur eosin methylene blue solution (109204, Merck, Darmstadt, Germany).
