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During 800 nm femtosecond laser irradiation the lenses emitted a strong blue autofluorescence signal indicating that two-photon absorption took place (Fig. 6).
The observation of blue autofluorescence indicates that the photobleaching was mediated by two-photon absorption of the infra-red laser light leading to ultraviolet equivalent effects in the focal region.
Blue: autofluorescence.
Blue: autofluorescence (A,F,H,I).
In addition, the mature phagolysosomal environment was found to have no effect of the blue autofluorescence.
The blue autofluorescence was detected in the 420 to 470 nm range.
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CPT was recognised by examining the characteristic crystal morphology and the light-blue autofluorescence that this compound emits under UV-light [ 14, 47].
Blue laser autofluorescence imaging revealed increased accumulation of autofluorescent storage material in the inner retinae of 7-month-old Ppt1 -/- and of 16-month-old Cln3 -/- mice in comparison with age-matched control littermates.
Blue laser autofluorescence fundus imaging faithfully revealed the typical accumulation of autofluorescent storage material in the inner retinal layers of both knockout mouse models.
The representative TUNEL (red) images merged with the DAPI (blue) and autofluorescence (green) images.
However, blue laser autofluorescence (BAF) fundus imaging revealed many spots of hyperfluorescence in Ppt1 -/- mice.
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