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The role of hemin trioxane interaction in the hemin-induced oxidative damage has been studied using methylene blue as a substrate by UV vis spectroscopy.
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This paper established a local provenance for the colouring agent of the blue glaze used as a substrate of gilding decorations.
However, this precipitate is more diffuse than the product of the reaction using nitro blue tetrazolium chloride (NBT /BCIP as a substrate.
-wrap-foot> aAnthraquinone dye bAzo dye cActivity was calculated as specific activity in U.mg−1 (1U = 1 μmol/min) dRelative activity was defined as activity toward Reactive Blue 19 Because the physiological substrate of TfuDyP is not known, we used Reactive Blue 19 as a representative substrate in subsequent experiments because of the high reactivity of the enzyme towards this dye.
Detection of bound DIG-riboprobes was achieved by means of alkaline phosphatase-conjugated to anti-DIG F ab fragments and 5-bromo-4-chloro-3-indolyl-phosphate/nitro blue tetrazolium as a chromogenic substrate (all reagents from Roche).
Bound Abs were detected with an alkaline phosphatase-conjugated secondary antibody and western blue as the substrate (Promega, USA).
EC-Blue (Medicago, Uppsala, Sweden) was used as a substrate for HRP and the signal was detected by measuring the absorbance at 450 nm.
Then, the dipstick was immersed in MC-1 activity buffer solution containing NADH as a substrate and nitrotetrazolium blue as the electron acceptor.
Hybridized signals were colored blue with 4-nitro blue tetrazolium chloride and 5-bromo-4-chloro-3-indolyl-phosphate as a substrate of alkaline phosphatase.
The normal in situ hybridisation protocol was then followed using anti-digoxygenin antibody conjugated to alkaline phosphatase and chromogenic reaction using nitro-blue tetrazolium chloride/5-bromo-4-chloro-3′-indolyphosphate p-toluidine salt (NBT/BCIP) as a substrate (Harland, 1991).
Strain ASU-06 showed an ability to produce indigo (blue) colored colonies as a result of dioxygenase activity in the presence of indole as a substrate.
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