Sentence examples for blotting were sequenced using from inspiring English sources

PCR products as used for reverse Northern blotting were sequenced using primer RaSH1R, which binds within pBluescript immediately upstream of the inserts to be analyzed [ 60].

Plasmids isolated were sequenced using Sanger sequencing.

Recombinants were sequenced before use.

All constructs were sequenced, and after plant transformation by floral dip70, single copy transgenic lines were identified by Southern blot analyses and representative lines were used for crosses and further analyses.

Sequencing and Northern blotting were respectively used to confirm deletion of these loci and the absence of their corresponding transcripts.

The dot blotting was performed using streptavidin-HRP.

Western blotting was performed using anti-Pfs25 4B7 mAb.

Northern blotting was done using standard techniques.

Western blotting was performed using PVDF membranes.

Blots were developed using Amersham chemiluminescence kit (GE Health lifesciences, US).

Blots were visualized using ECL (Amersham).