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The lysates for western blotting were generated using standard 1XRIPA buffer supplemented with 1X Protease Inhibitor Cocktail (Sigma).
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Dot-blots were generated using aliquots of input A. suecica chromatin, chromatin absorbed in the absence of antibodies ("beads only" control), or chromatin immunoprecipitated by antibodies specific for H3K4me3 or H3K9me2 and then captured on protein A beads.
Western blots were generated using standard procedures.
The membrane was initially incubated with 15 ml of pre-warmed hybridization solution (Ambion, cat 8670) for 1 hour at 65 C. Northern blot probes (32P) were generated using Amersham rediprime II random prime labeling system (RPN1633) according to the manufacturer's protocol (GE Healthcare, Piscataway, NJ).
Correctly targeted ES cells (Bmi1 fl-Neo ) were generated using W4 ES cells and identified by southern blotting (B ) using 5′ and 3′ probes.
Transgenic mice were generated using standard procedures.
The clusters were generated using Pearson correlation.
Figures were generated using SPDBV and PyMol.
Figures were generated using Pymol [45].
Merged images were generated using ImageJ software.
All structural representations were generated using VMD [31].
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