Northern blotting was done using NorthernMax formaldehyde-based system (Ambion Inc., Austin, TX) according to the manufacturer's recommendations.
Expression of HCL2-His6X wanalyzedzed on Tricine-SDS PAGE and Western Blotting was done using Anti-His6X antibodies.
The result of the Western blot analysis was scanned, and the quantification of the Western blotting was done using Quantity One imaging software (Bio-Rad, Hercules, CA, USA).
After immunoprecipitating with VDAC1 antibody (Abcam), western blotting was done using polyclonal antibodies against CRYAB and phospho CRYAB (Abcam), as described in the previous section.
Western blotting was done using PVDF membranes and a semi-dry transfer system, at 18 V for 15 to 20 minutes, with transfer buffer containing 0.1% SDS.
Western blotting was done using antibodies against following proteins: pYAP127 (1:1000, Cell Signaling Technologies); pYAP381 (1:1000, Cell Signaling Technologies); YAP (1 200, Sigma, St .Louis, Missouri); MLC (1 1000, Cell Signaling Technologies); SPTAN1 (1 500, Santa Cruz Biotechnology, Dallas, Texas); and beta-actin (1 200, Sigma).
Quantitative Western Blots were done using the Odyssey Infrared Imagines System (LI-COR Biosciences) using Alexa Fluor680 secondary antibodies (Molecular Probes).
Western blot were done using 30 µg of nuclear extracts and according to Perilhou et al. [7].
