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The GFP probe for Southern blotting was generated according to the instruction of DIG DNA Labeling and Detection Kit II (Roche, American) based on its sequence.
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Targeted Map3k1 mPHD/+ and Map3k1 mPHD ES cells were generated according to standard procedures (Gossler et al, 1986) and genotyped by Southern blotting or genomic PCR (Ledermann, 2000; Xia et al, 2000).
It is generated according to (10).
Data were generated according to Equations 2-5.
Crude cell extracts for western blotting were generated as described (Knop et al. 1999).
Western blots were generated from 2-D gels of both bovine pericardium protein fractions.
Western blots were generated with protein extracts from XIAP injected eyes and contralateral uninjected control eyes.
Western blots were generated using standard procedures.
Density blots were generated with R studio.
500 are generated abroad, according to FactSet.
Excess secondary antibody was rinsed off the membrane with TBST, and a chemiluminiscent signal was generated by using western blot detection reagents (ECL, Beyotime Institute of Biotechnology) according to the manufacturer's protocol.
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