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Northern blotting was done using standard techniques.
Northern blotting was done using NorthernMax formaldehyde-based system (Ambion Inc., Austin, TX) according to the manufacturer's recommendations.
Expression of HCL2-His6X wanalyzedzed on Tricine-SDS PAGE and Western Blotting was done using Anti-His6X antibodies.
After immunoprecipitating with VDAC1 antibody (Abcam), western blotting was done using polyclonal antibodies against CRYAB and phospho CRYAB (Abcam), as described in the previous section.
Western blotting was done using PVDF membranes and a semi-dry transfer system, at 18 V for 15 to 20 minutes, with transfer buffer containing 0.1% SDS.
The result of the Western blot analysis was scanned, and the quantification of the Western blotting was done using Quantity One imaging software (Bio-Rad, Hercules, CA, USA).
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Gel electrophoresis and blotting were done using an XCell tank (Invitrogen) according to the manufacturer's protocols.
Densitometric analysis of Western blots was done using the Kodak Gel Logic system.
Final imaging of the blot was done using the Fluor Chem Q system (Protein Simple, Santa Clara, CA).
Quantification of western blots was done using Multi-Gauge (Fuji-Film) and Image J (NIH).
Densitometric analysis of western blots was done using AlphaEase FC software (Alpha Innotech, Santa Clara, CA).
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