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Statistical significance in glucose uptake assays, glycogen synthesis assays, and Western blotting was determined using RM two-way ANOVA followed by the Bonferroni multiple comparison test.
Relative band density for Western blotting was determined using ImageJ gel analysis software.
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Immunodetection of the protein blotted was determined using a Fujifilm Intelligent Dark Box II.
The intensity of the bands in stained gels and blots was determined using Nonlinear.
Intensity of the blot was determined using the Image J software.
Intensity of the blot was determined using the Image J software (NIH, Bethesda, MD, USA).
Density of bands on film exposed to blots was determined using Scion Image software (Scion Corp., Frederick, MD, USA).
After incubation with second antibody (1 : 2500) for 1 h at room temperature, the blots were determined using an ECL Western blotting kit (Amersham Pharmacia Biotech, Buckinghamshire, UK), according to the manufacturer's instructions.
All results are reported as means±S.E.M. Statistical significance for ELISA, Western blotting and dot blotting data was determined using a two-tailed Student's t test.
Densitometric quantitation of blotting strips was determined using the Quality-One software.
HER-2 status was determined using Western blotting [ 27].
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