Sentence examples for blotting was conducted using from inspiring English sources
Protein (50 µg) was loaded onto 10% SDS-PAGE gels and western blotting was conducted using a primary antibody against ADMR (cat # LS-A4048 MBL International Corporation, Woburn, MA) at a 1: 1000 dilution and using a primary antibody against CRLR (cat # sc-30028, Santa Cruz Biotechnology, Santa Cruz, CA) at a 1: 100 dilution.
Western blotting was conducted using routine protocol.
Western blotting was conducted using standard protocols.
Western blotting was conducted using PVDF membrane (GE Healthcare, Amersham, UK).
Western blotting was conducted using antisera generated against an EspP C-terminal peptide (Szabady et al., 2005) or the HA (Y-11) peptide (Santa Cruz Biotechnology, Dallas, TX).
Lectin blotting was conducted using the appropriate biotinylated lectins diluted in PBS containing 1 mM calcium chloride and 0.05% Tween-20 (PBST).
Immuno-blotting was conducted using anti-WT1 (1 250, catalog no. M3561, DAKO, Glostrup, Denmark), p53 (1:2000, catalog no. PAb 1801, Abcam, Cambridge, UK) and p63 (1:2000, catalog no. M7247, DAKO, Glostrup, Denmark).
Quantification of the blots was conducted using Image-Pro Plusoftwarere (version 6.0; Media Cybernetics, Bethesda, MD, USA).
Densitometry of Western blots was conducted using Adobe Photoshop (Adobe Systems Incorporated, San Jose, CA, USA).
Western blot was conducted using a Multiphor II Electrophoresis System (GE Healthcare UK Ltd) at 200 mA for 1.30 h.
After the separation of the protein mixture, Western blot was conducted using the Trans-Blot® TurboTM Transfer System (Bio-Rad, USA).
