Your English writing platform
Discover LudwigSuggestions(3)
Exact(1)
To confirm the observations, the paired samples were analyzed for miRNA expression by northern blotting using a set of specific miRNA probes (Fig. 4B and 4C).
Similar(59)
The same samples were also analyzed by far-Western blotting using a limited set of SH2 probes (Suppl. Fig. S4B).
As a first step, the isolated tumor sets (primary tumor and metastases) were analyzed by Southern blotting using a MMTV-LTR specific probe.
Validated methods included pulsed field gel electrophoresis (PFGE), variable number of tandem repeats (VNTR) typing, and Southern blot hybridization using a set of MA DNA probes, including those for typing the vpma genes repertoire.
For example, Figure 1A shows blots using a combined set of 7 radiolabeled oligonucleotide probes, each 50 nts in length, that together encompass 687 nt of the 3' terminus of the clec-67 gene (Figure S1).
After washing, the bound proteins were analyzed by western blotting using an anti-Rac1 antibody.
To address the reviewer's concern in full, we added two new western blots using an alternative set of siRNAs against eEF1A1 (Pair B) to exclude potential off-target effect.
For this purpose, Southern blot analyses were first conducted using a set of 7 probes designated AGA/BOV that had been identified previously as specific for MA or Mycoplasma bovis but that had also been found to be subject to inter-strain variation within a species (Additional file 2: Table S2).
We calibrated the method using a set of manually-designed genomic probes that we have previously successfully employed for Southern blotting (see Table 1).
For Western blot, samples were loaded onto a NuPAGE® Novex® 4 12% Bis-Tris Gel (Life Technologies) and blotted using in an iBlot Gel Transfer Device (Life Technologies).
Since 2009, in-house Western blot has been done by using a commercial set (LDBIO, Lyon, France).
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com