Sentence examples for blotting using a series from inspiring English sources

Peptide blotting using a series of designed peptides with profilins 1 and 2a indicates differences between the two profilins towards proline-rich peptides from mDia1 and VASP.

We used the ZNF10 KRAB domain as a positive control because the KRAB domain of this factor has been shown to interact with KAP1 in vitro [31]; unfortunately, we could not detect this protein by western blot using a series of commercially available antibodies (data not shown) and therefore we could not test if ZNF10 recruits the KAP1/SETDB1 complex in cells.

The abundance of TR families was estimated by quantitative dot-blot analysis using a series of genomic DNA dilutions ranging from 50 to 200 ng.

After washing, the bound proteins were analyzed by western blotting using an anti-Rac1 antibody.

For Western blot, samples were loaded onto a NuPAGE® Novex® 4 12% Bis-Tris Gel (Life Technologies) and blotted using in an iBlot Gel Transfer Device (Life Technologies).

To additionally ensure that the quantified western blot signal is within the linear range of detection, we generated standard curves using a dilution series of the respective ubiquitination reaction, which was incubated for 180 min at 30 °C.

a, Immunoprecipitation of endogenous METTL3 and western blotting using the indicated antibodies.

d, Western blotting using the indicated antibodies.

An anti-Myc antibody was used to precipitate Rac1, and western blotting was using an HA-specific antibody.

An anti-Myc antibody was used to precipitate the lysates, and western blotting was using an anti-HA antibody.

When cleaning your mattress, make sure to blot the stain using a damp white cloth and cold water.