Sentence examples for blotting using a pan from inspiring English sources

Brk kinase activity as measured by Brk autophosphorylation and phosphorylation of its substrate, Sam68, was determined by Western blotting using a pan antiphosphotyrosine (4G10) antibody.

Beads were then boiled in SDS PAGE sample buffer for 3 min and analyzed by SDS PAGE and Western blotting using an anti-His mAb (Novagen) or the pan-specific Skp1 mAb 4E1, and Alexa-680-conjugated rabbit anti-mouse IgG.

Since antibodies for the different CDK substrates (cyclins) are not available, we set out to validate these findings by using a pan CDK p-substrate western blot.

If using a pan, smooth the mixture into the pan until it is evenly distributed.

Levels of SPRY were analysed by Western blotting in control and ccp1 expressing MEF using a pan-SPRY antibody followed by densitometry analysis.

Samples were analyzed by gel filtration as described above or SDS PAGE followed by staining with Coomassie Brilliant Blue or Western blotting using the pan-specific Skp1 mAb 4E1 and Alexa-680-conjugated rabbit anti-mouse IgG.

The MW of the phosphorylated PKC enzyme detected by Western blotting using the pan-specific PKC antibody was in the range 76 82 kDa suggesting the relevant isoform was either PKC δ (78 kDa), PKC θ (76 kDa) or PKCα/β (80 82 kDa).

Use a pan that is roughly 13x9".

Alternately, you can use a pan or a skillet.

Use a pan that you would make brownies in.

Do not use a pan that isn't flat.