Sentence examples for blotting using a mouse from inspiring English sources
We digested previously isolated lambda genomic clones of the human FGF8 region [ 22] with restriction endonucleases (Promega, Madison, WI) and analyzed them by Southern blotting using a mouse Npm3 cDNA [ 21] probe, as previously described [ 20].
The expression of the HA proteins was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) under reducing and non-reducing conditions and western blotting using a mouse monoclonal anti-V5 antibody (Invitrogen), or a rabbit polyclonal anti-H5N1 HA1 specific sera.
6X-His tagged CspA, RhlB, SrmB, and CsdA were detected by Western blotting using a mouse monoclonal anti-6X His tag antibody (Abcam, United Kingdom) followed by a goat anti-mouse polyclonal antibody conjugated to HRP (Abcam).
To determine if human NPM3 is similarly located and to identify genomic clones of NPM3, we analyzed FGF8-containing human genomic lambda clones [ 22] by Southern blotting using a mouse Npm3 cDNA probe.
Equal loading of protein was determined by Western blot using a mouse anti-β-actin antibody (Novus Biologicals).
Active Rac1 (GTP bound) in the supernatants was detected by western blot using a mouse anti-Rac1 mAb (Cell Biolabs).
The product demonstrated as a 17- and a 34-kDa band; both bands were identified by western blot using a mouse anti-hIL-25 monoclonal antibody.
Caspase-mediated cleavage of PARP was assessed by western blot using a mouse monoclonal anti-human PARP (BD Biosciences) at 1 : 250 dilution.
Then, RAS protein was assessed by western blot using a mouse anti-RAS clone10 antibody (1 : 1000) in GST pull-down and whole lysates (Millipore-Upstate, Billerica, MA, USA) and a goat anti-GST antibody (1 : 1000) or a mouse α-tubulin antibody (1 : 5000) (GE Healthcare), respectively.
M45- or HA-tagged SipA, SopE or SptP present in the bacterial cell pellet was detected by Western blotting using a monoclonal mouse-α-M45-, or a polyclonal rabbit-α-HA antibody and the signal was calibrated by comparison with known amounts of purified SipAM45 or SipAHA fusion proteins present on the same blot (see supp. Fig. S1).
Cells (10) were heated in reducing SDS-sample buffer (50 μL), subjected to SDS/PAGE and AID abundance monitored by Western blotting using biotinylated mouse anti-hAID mAb h52 1 18 developing with SA-HRP (GE Healthcare).
