Sentence examples for blotting using a flanking from inspiring English sources

Deletion of the genomic sequence was confirmed by Southern blotting using a flanking probe as well as a neomycin probe (Figure 4B).

Currently, the number of D4Z4 repeats is usually determined by Southern blotting using a probe that hybridizes to the centromeric flanking sequence, p13E-111.

Targeting events were identified by insertion-specific PCR and confirmed by Southern blot using a flanking probe.

Approximately 300 recombinant clones were screened by Southern blotting using a PCR generated 3′ flanking probe in order to confirm the site-specific integration of the LoxP sites (Fig. 1A).

After washing, the bound proteins were analyzed by western blotting using an anti-Rac1 antibody.

For Western blot, samples were loaded onto a NuPAGE® Novex® 4 12% Bis-Tris Gel (Life Technologies) and blotted using in an iBlot Gel Transfer Device (Life Technologies).

Correct targeting was confirmed in 5.2% of all embryonic stem cell clones by Southern blotting using flanking 5' and 3' probes and a NeoR internal probe.

Southern blots using flanking probes from outside the targeted regions confirmed the presence of the insert (Fig 1D).

a, Immunoprecipitation of endogenous METTL3 and western blotting using the indicated antibodies.

d, Western blotting using the indicated antibodies.

An anti-Myc antibody was used to precipitate Rac1, and western blotting was using an HA-specific antibody.