Sentence examples for blotting procedures were used from inspiring English sources

Routine Western blotting procedures were used to detect protein content as described10.

Standard western blotting procedures were used as previously described [ 8].

Routine Western blotting procedures were used to detect protein content as described previously [ 30].

Proteins (30 μg/sample) were separated via reducing 10% SDS-PAGE and standard western blotting procedures were used to detect proteins of interest with the following primary antibodies: PDK2 (Cat# AP7039b, Abgent, San Diego, CA, USA), β-actin (Abcam), Noxa (OP180, Calbiochem) and Puma (ab9643, Abcam).

The same SDS-PAGE and western blot procedures were used to directly detect proteins in 15 µg of total extract prepared in NET-Tx100 lysis buffer or in 1 µg of raft proteins prepared in 6% RIPA buffer.

For Western blotting, standard immunoblotting procedures were used [68].

Similar procedures were used for Western blot expression assessment of TGF-β1 (anti-TGF-β1, Santa Cruz, Biotechnologies, Santa Cruz, USA), phospho-Smad (anti-Smad2, phospho-specific, Calbiochem, Schwabach, Germany) and phospho-ERK (anti-active ERKMAPK, Calbiochem, Schwabach, Germany).

The rest of Western blotting procedure was performed according to the standard method using horseradish peroxidase-conjugated anti-rabbit antibody for protein detection with the ECL assay system (GE Healthcare) [30].

Standard western blotting procedures were followed using Tris-glycine SDS-PAGE and electrotransfer onto PVDF membrane at 100 V for 1 h at 4 ºC.

The phage libraries were titred to obtain approximately 500,000 pfu per plate and blotting procedures were performed in duplicate using Nylon membranes (Hybond-N, GE healthcare, UK).

SDS-PAGE and Western blotting procedures were performed as described [19], chemiluminescence using CDP-Star (Roche) or ECL Western blotting detection reagents (GE Healthcare) was detected with Fuji Medical X-ray films (Fujifilm).