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To evaluate the system, five cDNA clones from a mouse testis cDNA library were expressed, and two alternative blotting procedures were developed for convenient screening of expression efficiencies.
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Specific consent procedures were developed.
The chromatographic procedure was developed.
Routine Western blotting procedures were used to detect protein content as described10.
Standard Western blotting procedures were followed.
Standard western blotting procedures were employed.
Standard western blotting procedures were used as previously described [ 8].
The details of Western blotting procedures were described previously [ 30].
Southern blotting procedures are described in Supplemental Data.
Blots were developed using ECL procedures.
All blots were developed using ECL Western blotting kit (GE Healthcare, Munich, Germany).
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