Sentence examples for blotting procedures were applied from inspiring English sources

Regular Western blotting procedures were applied to the samples, with monoclonal anti-mSlo3 antibody (1∶200, Antibodies Inc) or monoclonal anti-mβ4 antibody (1∶200, Antibodies Inc) as the primary antibody and the HRP-conjugated goat anti-mouse IgG antibody (1∶1000, Pierce) as the secondary antibody, correspondingly.

Validation procedures were applied.

Standard Drosophila husbandry procedures were applied.

Then, different post fixation procedures were applied.

Standard procedures were applied.

Two procedures were applied.

Standard immunohistochemical procedures were applied.

Again, the same staining procedures were applied.

For Western blotting experiments on SD, H2O2, SD + H2O2, TNF or SB treated rMC-1 cells (Additional file 2: Figure S2), the same procedures were applied except for the detection system and quantification method.

Minimal surgical procedure was applied.

A standard procedure was applied.