Sentence examples for blotting method using a from inspiring English sources
The methylation states were analyzed by Southern blotting method using a methylation-sensitive enzyme, HpaII, and a partially methylation-sensitive isoschizomer, MspI.
After protein quantitation using the Lowery protein assay, equal amounts of proteins were separated by SDS-PAGE and blotted onto nitrocellulose membranes by the semi-dry blotting method using a three-buffer system.
For specific HEV antibody detection in swine, we developed ELISA and Western blotting methods, using a truncated capsid (ORF2) protein lacking the first 111 amino acids of a swine HEV genotype 3 strain.
Patterns of recognition of brain proteins by plasma IgG were detected by quantitative immunoblotting (PANAMA-blot method), using a protein extract from the brain of a healthy individual as the source of antigens as previously described [26], [27].
The semi-quantitative western blotting method using synthetic mouse sarcolipin was validated by estimating the levels of sarcolipin in the atria of FVBN mice.
The results of the genotyping data were consistent with the dot-blot-SNP method using a single SNP marker.
We examined the expression of pancreatic secretory trypsin inhibitor (PSTI) in colorectal cancer by immunohistochemical staining using an anti-PSTI antiserum, an in situ hybridisation technique utilising sulphonated PSTI cDNA probe, and a Northern blot hybridisation method, using a 32P-labelled PSTI cDNA probe.
After four times washes with lysis buffer, the precipitated materials were analyzed by western blot method using the indicated Abs.
When using the Immunetics ELISA as a screening tool, it becomes clear that, irrespective of the blot method used, samples with an index >4 are almost always blot-positive.
Thus, taking into account that the only available data were related to the amount of monomers, dimers and the sum of all oligomers of ASYN (restricted by the western blot method used to detect them), part of the system could not be directly parameterized.
Western blots were performed using standard methods using a Reinhard lysis buffer supplemented with protease and phosphatase inhibitors (Roche, Basel, Switzerland).
