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Levels of H3K4me3 were determined by western blotting assay using a specific antibody.
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To determine the downregulating effects of miR-19a/b at the protein level, we performed western blotting assays using an anti-MXD1 antibody.
Our western blotting assay using 6E10 and E50 revealed that Aβ A2T and C99 A2T were transferred onto nitrocellulose, as were Aβ WT and C99 WT.
Western blotting assays using both anti-GST and anti-glucagon antibodies confirmed the presence of the GST-fused glucagon (Figure 1B).
The oligomeric structure was observed as a low mobility 250-kDa band in a Western blot assay using a specific anti-Cry1Ab antiserum.
Western blot assay using a Flag antibody indicated that all suppressors are expressed at a high level in the progenies from these crosses (Fig. S1).
To determine if the stained 140 kDa and 90 kDa bands correlated with direct dengue 3' SL RNA binding potential, a northwestern blot assay, using a radiolabeled dengue 3' SL RNA probe, was performed.
Mucin in perfusates was quantified by periodic acid-Schiff's base dot-blot assay, using a kit (Sigma; catalog 395B).
The presence of the scaffoldin on the cell surface was assessed by dot-blot assay using a specific antibody against the CBM (Additional file 5: Figure S5).
The expression levels of the p53 and p21 proteins were determined by western blot assay using a specific anti-p53 or anti-p21 antibody.
Enzymatic activity from the supernatant of the infected cells was demonstrated by dot blot assay using an antibody that recognizes the digested form of CSPG and was compared with the bacterial ChABC enzyme.
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