Sentence examples for blotting analysis were employed from inspiring English sources

The XTT (2,3-bis [2-methoxy-4-nitro-5-sulfophenyl]-2H-tetra-zolium-5-carboxanilide) assay for cytotoxicity, the ELISA, RT-PCR and western blotting analysis were employed in MCF-7 human breast cancer cells under hypoxic conditions.

The XTT (2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide) assay for cytotoxicity, ELISA (enzyme linked immunosorbent assay), RT-PCR and Western blotting analysis were employed in MCF-7 human breast cancer cells under hypoxic conditions.

Western blotting analysis was employed to confirm the alteration in expression at the protein level.

Quantitative real-time polymerase chain reaction arrays and Western blot analysis were employed to identify the cell-cycle control- and apoptosis-related genes regulated by CENP-A.

SDS-PAGE and Western blot analysis were employed to determine the enrichment of serum.

RT Q-PCR and Western blot analysis were employed to investigate the activities of AMPK, FOXM1 and AKT/FOXO3a signaling.

Spectrofluorimetry, circular dichroism (CD) studies, in vitro histone acetyl transferase (HAT) assay and western blot analysis were employed as experimental tools.

Real-Time PCR and Western blot analysis were employed to validate the changes in expression of relevant genes including the protein levels of C3 which is a component of the complement cascade.

In addition, both IHC and Western blot analysis were employed to evaluate levels of derlin-1 expression in another set of tumor samples with paired normal breast tissues from 13 patients.

Western blot analysis was employed to quantify total numbers of hippocampal NR2A and NR2B.

Western blot analysis was employed to evaluate levels of AMPK, mTOR, raptor, Beclin-1, p62 and LC3-II.