Sentence examples for blotting analysis using the from inspiring English sources

They were then subjected to proteinase K digestion followed by Western blotting analysis using the Sha31 anti-PrP antibody [40].

Western blotting analysis using the cell lysates of U937 and HEK293 cells, showed several bands detected by anti-gastric H+/K+-ATPase α antibody (data not shown).

The eluted proteins were resolved on 10% SDS-PAGE gel electrophoresis for western blotting analysis using the indicated antibodies.

To assess whether PBC patients produce autoantibodies to the hmAchR of the M3 type, we next performed a Western blotting analysis using the membrane fraction of Sf9 cells over-expressing the recombinant hmAchR receptor protein (rhM3R) of the M3 type.

Cell lysates from ARV S1133-infected cells with or without Y-27632 treatment were immunoprecipitated with anti-ROCK1 or anti-Beclin-1, then subjected to Western blotting analysis using the indicated various antibodies.

Total proteins of the leaves were extracted in 1x Laemmli buffer (2.5 mM Tris-HCl, pH 8.3, 250 mM glycine and 0.1% SDS) and incubated in boiling water for 5 min. Proteins separated by SDS-PAGE were subjected to Western blotting analysis using the polyclonal rabbit anti-BaMV coat protein antibody.

Then, isolated protein samples were subjected to 10% SDS-PAGE separation and subsequent Western blot analysis using the indicated antibodies.

The molecular weight of coPEDF was confirmed by western blot analysis using the conditions of Laemli.

The mRNAs were detected by routine western blot analysis using the anti-T7 antibody.

Levels of different proteins were analyzed by Western blot analysis using the respective antibodies.

The degree of Proteinase K digestion was visualized by Western blot analysis using the anti-FLAG antibody (Sigma).