Sentence examples for blotting analysis using an from inspiring English sources

After 30 hours, the whole cell lysates were subjected to the GST-PAK pull down assay, followed by western blotting analysis using an anti-Myc antibody.

Then, the cell lysates were collected and subjected to the GST-PAK pull down assay followed by western blotting analysis using an anti-Rac1 antibody.

The intracellular expression levels of GAD in the engineered C. glutamicum strains were monitored by western blotting analysis using an antibody raised against the FLAG-tagged sequence that was incorporated into the cloned genes.

The expression of the LeGFP protein was determined by Western blotting analysis, using an anti-GFP antibody.

Furthermore, Western blotting analysis using an anti-cytochrome c antibody showed that Cyc1 disappears from the mitochondrial extracts with a kinetics very similar to its loss of peroxidase activity (as detected by heme staining) (Fig. 3-A).

DOI: http://dx.doi.org/10.7554/eLife.05061.020 For further validation of these results, we performed Western blotting analysis using an antibody targeting the human orthologue of Ntf3.

Western blotting analysis using a phospho-specific Hand1 antibody also showed Hand1 to be phosphorylated at both T107 and S109 (Figure 3C).

Western blotting analysis using a monoclonal anti-phosphoserine antibody demonstrated the phosphorylation of Δ148aa-hACC2 Δ148aa-hACC2m silkworm purifiedig.  4a).

(a) Northern blotting analysis using Actin2 as a reference.

(a) Northern blotting analysis using Actin2 as a reference gene and PM protein immunoblotting with antibodies specific to the indicated proteins.

The cells were then lysed for Western blotting analysis using a rabbit anti-LC3 antibody to detect the endogenous LC3 expression (top panel in each Fig. 2C E).