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Western blotting analysis using a phospho-specific Hand1 antibody also showed Hand1 to be phosphorylated at both T107 and S109 (Figure 3C).
At 4 dpi with each recombinant virus, the recombinant GFP protein in the inoculated leaves of Samsun nn was detected by western blotting analysis using a rabbit antibody to GFP (Figure 2B).
Western blotting analysis using a monoclonal anti-phosphoserine antibody demonstrated the phosphorylation of Δ148aa-hACC2 Δ148aa-hACC2m silkworm purifiedig. 4a).
Protein expression was assessed by western blotting analysis using a primary antibody directed against the Myc tag (monoclonal mouse anti-myc antibody 9E10 (1 100), from Invitrogen) and as a secondary antibody a horseradish peroxidase-labelled goat anti mouse antibody (Sigma-Aldrich) was used.
The cells were then lysed for Western blotting analysis using a rabbit anti-LC3 antibody to detect endogenous LC3 expression (top panel in Fig. 3A).
The cells were then lysed for Western blotting analysis using a rabbit anti-LC3 antibody to detect the endogenous LC3 expression (top panel in each Fig. 2C E).
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After 30 hours, the whole cell lysates were subjected to the GST-PAK pull down assay, followed by western blotting analysis using an anti-Myc antibody.
Then, the cell lysates were collected and subjected to the GST-PAK pull down assay followed by western blotting analysis using an anti-Rac1 antibody.
The expression of the LeGFP protein was determined by Western blotting analysis, using an anti-GFP antibody.
(a) Northern blotting analysis using Actin2 as a reference.
(a) Northern blotting analysis using Actin2 as a reference gene and PM protein immunoblotting with antibodies specific to the indicated proteins.
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