Sentence examples for blots were treated with from inspiring English sources

Blots were treated with 5% skimmed milk for 1 h at room temperature and then incubated with a rabbit polyclonal anti-Stx1 antibody (Cat No. 64-025, BiOsaka, Japansaka, Japan) or a mouse monoclonal anti-Stx2 antibody (Cat No. 20273-04, Nakalai Tesque) in PBS containing 0.1% Tween 20 (PBS-T) and 0.5% skimmed milk for 1 h at room temperature.

Blots were treated with the rabbit anti- OsLIC serum (1∶200).

Following the final wash, blots were treated with ECL and exposed to x-ray film.

After washing, the blots were treated with secondary antibodies conjugated to horseradish peroxidase, and developed with chemiluminescence Western blotting kit (Roche) or ECL plus (Amersham).

In brief, blotting was done by the submarine-type mini-transblot Bio-Rad Laboratoriess, Tokyo) onto PVDF membrane (PALL, FL) and the blots were treated with 5% skim milk (Nacalai Tesque, Kyoto) for blocking.

All blots were treated with Lumigen PS-3 detection agent.

After exposure of the ghost proteins on the membrane to these three different antigens the blot was treated with anti-RH5 (which contains antibodies to GST too) to detect specific ligand-receptor interactions.

The upper part of the blot was treated with mouse anti-HSP70 antibody and the lower part was treated with mouse anti-actin antibody.

Proteins were electro-transferred to the PVD membrane, and the blot was treated with biocytin hydrazide (BCHZ) to label glycoproteins (see Materials and Methods for details).

After the detection of autophosphorylated CaMKII, the blot was treated with Blot Restorte Membrane Rejuvenation Kit (Millipore) according to the manufacturer's instructions, so that it could be reprobed.

To confirm equal loading, the blot was treated with Restore western blot stripping buffer (Thermo Fisher Scientific, Hemel Hempstead, UK) for 15 min at room temperature, washed, blocked and re-probed with an antibody to human β-actin.