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Blots were then stained with Ponceau S and photocopied.
Blots were then stained with a horseradish peroxidase-conjugated goat anti-mouse secondary antibody (DakoCytomation, Ely, Cambridgeshire, UK) and detected by the enhanced chemiluminescence method (Amersham).
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To ensure equal spotting of total DNA on the membrane, the same blot was then stained with 0.02% methylene blue in 0.3 M sodium acetate (pH 5.2).
Separated proteins were then stained by immune blot analysis using anti-Hsp90 serum.
The membranes were then stained with Coomassie blue, a general protein-staining dye, after Western blotting to visualize the major proteins in fly heads.
The gels were then stained with Coomassie Brilliant Blue G-250 (BioRad) or the proteins were blotted onto low-fluorescent Hybond-LFP PVDF membranes (Amersham).
These slides were then stained with Giemsa.
Sections were then stained with hematoxylin-eosin.
Chips were then stained using streptavidin phycoerytherin.
The sections were then stained (Van Gieson).
Cells were then stained.
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