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Western blots were then quantified with scion image software.
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The images were then quantified by Image J.
The western blotting signal was then quantified by morphodensitometric analysis using ImageJ software (NIH, Bethesda, MD, USA).
In vitro phosphorylated c-Jun was then quantified by Western blotting using antibodies specific for phosphorylated substrate.
The increased KIF5 in the secretory vesicle fraction was then quantified by western blotting (Fig. 3E,F).
The amount of AKT in each lysate was then quantified by Western blot using an HA antibody and the lysates normalized for AKT content using image analysis of AKT immunoreactive bands.
The VCDR was then quantified.
Luciferase activity as bioluminescence was then quantified.
Fluorescence is then quantified using flow cytometry.
Reduced MTT was then quantified photometrically.
Immunoreactive RhoA or Rac1 were then quantified by western blot analysis.
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