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The blots were then exposed to X-ray film and band positions and band densities were calculated using a computerized image analysis system (UVP Biochem Imaging systems, Upland, CA).
Blots were then exposed to radiographic film.
Blots were then exposed to X-ray film to obtain the fluorographic images.
The blots were then exposed to X-ray film for 60 s (Denville Scientific Inc, South Plainfield, NJ).
Blots were then exposed to autoradiographic films and developed with the ECLPlus Western Chemiluminescent Detection System (GE Healthcare, Amersham) to determine levels of protein expression.
The blots were then exposed to hyperfilm and the intensity of the protein signal was determined from the digitized film images with Gel Expert Software 4.0.
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The blot was then exposed on x-ray film (AGFA Curix RPI Agfa HealthCare NVV, Mortsel, Belgium) to obtain an image.
The blot was then exposed to an X-ray film and developed using standard procedures.
Blot was then exposed to Kodak XAR film (Eastman Kodak, Rochester, NY, USA).
The blot was then exposed to primary antibody, caspase-3 rabbit polyclonal IgG, Santa Cruz, USA (sc-7148) at 1 1000 dilution.
The blot was then exposed to an X-ray film (Amersham) in order to obtain the image.
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