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The blots were then analyzed with a Fujix Bas 2000 phosphoimager (Fuji Photo Film, Tokyo, Japan).
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Cells were treated with 5 mM NAC for 2 h before and during 12 h exposure to fluvastatin, cell viability, western blotting and DNA fragmentation were then analyzed.
Partially purified Kv3.1 samples were then analyzed by Western blots (Figure 1).
Precipitated proteins were then analyzed by Western blots using anti-xMELK and anti-RACK1 specific antibodies.
Confluent cells from 17, 20 and 22 week primary fetal leptomeningeal cell cultures (L1-3), 2 WHO grade I (M2 -M 3) and 2 WHO grade II primary meningioma cultures (M 4 and M5) were serum deprived overnight then treated with serum free DMEM, cerebrospinal fluid without or with 250 nM cucurbitacin I. Lysates of the cells were then analyzed by Western blots.
The protein precipitates were then analyzed by SDS-PAGE and western blot, or dot blot in the case of GM1.
The purified HER2 proteins were then analyzed using Western blot, mass spectrometry and circular dichroism (CD) spectroscopy.
Proteins were then analyzed by western blot.
They were then analyzed by western blotting for GRK2 expression.
The digest samples were then analyzed using Western blotting with a GFP antibody (Fig. 14B).
Cells were then analyzed by western blot for the presence of TAF-like protein expression.
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