Sentence examples for blots were subsequently washed from inspiring English sources

The blots were subsequently washed with TBST and incubated with respective secondary antibodies.

The blots were subsequently washed three times (10 15 min at RT) in TBST and once in TBS (5 10 min).

The blots were subsequently washed with TBS containing 1% Tween 20 for 4 times and then incubated with HRP-labeled goat anti-rabbit secondary antibody (1∶2000) (Boster, China) for 2 hours at room temperature.

For detection of IgG, the blots were subsequently washed in PBST and then incubated with rabbit anti-human IgG (diluted 1∶3000) (BIO-RAD, Hercules, CA) for one hour at 37°C.

Blots were subsequently washed 5 minutes in AP-buffer (0.1 M Tris Base, 0.1 M NaCl, pH9.5) and signal was detected on X-Ray film using CDP-star kit (Roche #12041677001) according to manufacturer's instructions with a typical exposure time of 4 hours.

The blots were subsequently washed and treated with appropriate secondary antibodies.

The blot was subsequently washed 3× for 30 minute at RT with TBST followed by incubation with an anti-mouse-HRP secondary antibody (400 μg/ml dilution in TBST; Pierce, Rockford, IL) for 2 h at RT.

The blot was subsequently washed by 3 incubations with 1 mM methionine and 0,25% fatty-acid free BSA for at least 15 min, dried and binding quantified by phospho-imaging (Reader FLA 3000, Fuji-Film, Tokyo, Japan).

Spots identified by western blot were mapped back to and picked from the duplicate 2D gel, were subsequently washed and digested with trypsin (Promega, Madison, WI, USA).

Membranes were subsequently washed in PBS-T and developed using chemiluminescence western blotting detection reagents (Pierce Biotechnology).

Cells were subsequently washed with flow buffer.