Sentence examples for blots were subsequently probed from inspiring English sources

The blots were subsequently probed with anti-phospho-ser32/36-IκB-α anti-phospho-ser32/36-IκB-α anti-phospho-ser32/36-IκB-α anti-phospho-ser32/36-IκB-α anti-phospho-ser32/36-IκB-α

After gel electrophoresis, blots were subsequently probed with primary antibodies (anti- IL-6, 1∶1000 #ab6672, antIL-17-1∶30003000 #ab40663, anti-TGF-β 1∶1000 #ab66043 Abcam Inc; Cambridge, MA).

Blots were subsequently probed with Clean-Blot IP-horseradish peroxidase conjugate (Thermo Scientific).

Blots were subsequently probed with anti- α-tubulin (Sigma, Poole, UK) to show equal sample loading.

The same blot was subsequently probed with β-actin, washed, and exposed to Kodak MS film for 2 hours.

These fractions were subsequently probed for LDH1.

These blots were subsequently re-probed with an antibody against total ERK and p38 (New England Biolabs, Inc .. Bands were visualised as before.

Blots were subsequently stripped and probed using an antibody that recognises total STAT5 to verify equal loading of samples.

Blots were subsequently blocked and re-probed with a mouse anti tubulin antibody (0.15 μg/ml; Abcam, Cambridge, UK, ab11323).

For internal control of loading, the blots were subsequently hybridized to a glyceraldehyde-3-phosphate dehydrogenase (G3PDH) probe (Clontech, Mountain View, CA).

After probed with 1 500 diluted mouse anti-vimentin (Santa Cruz Biotechnology, Santa Cruz, CA, USA) at 4°C overnight, the blots were subsequently incubated with HRP-conjugated secondary antibody (1 5000).