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Blots were subsequently analyzed by Quantity One software (Biorad, Hercules, USA).
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The samples were subsequently analyzed by Western blot (Chen et al., 2010; Xiang et al., 2007).
The immunoprecipitates were subsequently analyzed by western blotting with an anti-HA (ubiquitin) to detect ubiquitylated TACC3 proteins.
Samples containing 75 μg of total protein were subsequently analyzed by western blot as described previously [ 17], using a primary anti-SRAP rabbit polyclonal antibody (cat # A300-743A, BethyLaboratorieses, Montgomery, TX, USA) raised against the C-terminal extremity of SRAP (peptide 180-237 aa).
Thus, rOat3 expressions were subsequently analyzed using qPCR and western blotting as described above in each respective materials and methods section.
Nuclear extracts were subsequently analyzed for p65-NF-κB and pSTAT-1α content by Western Blot analysis.
Samples from foaming digestates were subsequently analyzed.
Optical and damage properties were subsequently analyzed.
Only task-free trials were subsequently analyzed.
All samples were subsequently analyzed by SDS-PAGE.
Cells were subsequently analyzed as above.
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