Sentence examples for blots were stripped using from inspiring English sources
Afterwards, the blots were stripped using Stripping buffer (Pierce, Rockford, IL) and probed with anti β-actin antibody at 1 5000 dilution.
For successive antibody probes, blots were stripped using Pierce Restore™ stripping reagent with modifications to the manufacturer's protocol.
To ensure equal protein loading and transfer, the blots were stripped using the Blot Restore kit according to the manufacturer's instructions (Millipore; Billerica, MA), reprobed with mouse anti-β-actin antibodies and processed as stated above.
Blots were stripped using Restore Western Blot Stripping Buffer (Thermo) and re-probed with Tetra-HIS antibody.
Blots were stripped using β-mercaptoethanol as described previously (45) and reprobed for β-actin as a loading control.
The blots were stripped using Restore stripping buffer (Thermo Scientific) and re-probed using the above protocol with β-actin as loading control.
The blot was stripped using GM Biosciences One-Minute Western Blot Stripping Buffer (GM 6001), and the western protocol was repeated using RAD50 (GeneTex, GTX70228; 1:5000) as the primary antibody.
The blotting membranes were stripped using 65 mM Tris HCl, 100 mM 2-mercaptoethanol, 2% SDS for 5 min at 55 60°C, rinsed three times with PBST and thereafter incubated with the next primary and secondary antibodies.> Pieces of intestine and liver tissue were fixed in formaldehyde, dehydrated and embedded in paraffin.
Blots were developed with SuperSignal West Pico Chemiluminescent Substrate Piercee) and membranes were stripped using Restore Western Blot Stripping Buffer (Pierce).
Phospho-antibody blots used antibodies at 1 1000 and were stripped using Gentle Review Buffer (Amresco, Solon, OH) prior to incubation with total-AKT or total-ERK1/2 antibodies, also at 1 1000.
When necessary, PVDF membranes were stripped using Restore PLUS Western blot stripping buffer (Thermo Scientific, Rockford, IL) for 15 min at room temperature, washed twice in TBST, and then reprobed.
